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Ozone Therapy in Practice

Ministry of Health Service of The Russian Federation

The State Medical Academy of  Nizhny Novgorod

Keywords: Ozone, Discovery of Ozone, Fundamentals, Clinical effects, Ozone therapy.


“Ozone therapy has found its way into medical practice in Germany, where it is successfully
used. Germany was the first country to start manufacturing medical ozonators and to use
ozone-oxygen mixtures in vascular surgery, stomatology and geriatrics. Italian ozonetherapists have focused their activity on medical cosmetology. Specialized ozone therapeutic
clinics operate in Switzerland and other countries of West Europe. Cuba is well-known for its
Ozone Research Center. Interesting and promising results with the help of medical ozone
have been received in some clinics of the USA, Mexico, Brazil and Japan.

Ozone therapy was found to be efficient, easy to use, ensuring good tolerance and no sideeffects.
According to the chosen therapeutic concentration ozone can produce its immune-modulating,
anti-inflammatory, bactericidal, virucidal, fungicidal, analgesic and other effects.

Medical ozone proves to be of great therapeutic potential for in numerous cases it exceeds the
resources of medication-based methods. The procedures of its application are simple,
economically preferable and beneficial. However, medical communities and practical health
service still prefer not to notice the available convincing facts and evidences that might bring
it into wide practice.” (1)

Fundamentals of Ozone Therapy

“Ozone is known to be one of the most important gases in the stratosphere. It acts as a screen
for ultraviolet radiation of short wavelength of 260-280 nm, thus protecting the living
organisms on earth and absorbing infra-red radiation coming from the earth and, hence,
preventing its cooling.” (1)

“Ozone is highly reactive chemical element. It is constantly formed as a colourless gas at about
20-50 km above the surface of the earth under the influence of vacuum ultraviolet (UV) light
from atmospheric oxygen:

3O2 -> 2O3

And on the contrary, a molecule of ozone can absorb a particle of UV so producing diatomic
oxygen again. Ozone molecules dissociate into their constituent atoms These atoms combine
with oxygen molecules to form ozone and as a result of this process ozone layer –
ozonosphere is formed, which acts as a shield from the sun’s ultraviolet radiation, maintaining
the biological balance in biosphere.” (1)

Discovering ozone and ozone properties 

“Ozone as a chemical element was discovered at the end of XVIII century. In 1785 Martinus
Van Marum, a Dutch physicist, subjecting oxygen to electrical discharges noted some specific
“odor of electrical matter”. In 1848 C.Schonbein, a German physicist, having repeated the
experiments, named the smelling gas Ozone (from the Greek “ozone”-odorant) and described
some of its properties.” (1)

“For water purification ozone has been used for about 80 years. In Paris they use ozone-treated
water. With its high oxidative potential ozone is used in textile and cellulose industry as a
bleaching means. Ozone bactericidal properties are successfully used in breeding oysters and
mollusks as well as in mineral water industry.” (1)

Clinical Effects of Ozone Therapy 

“Ozone can produce different effect according to the chosen concentration and the way of its
administration. In medical practice the most important are the following ones.”(1) Ozone being used as a and to produce a:

1. Bactericidal, fungicidal and virucidal

2. Anti-inflammatory effect

3. Analgesic effect

4. Detoxication effect

5. Activation of oxygen-dependent processes

6.Optimization of pro- and anti-oxidant systems 

7. Haemostatic effect (depending on dosage) 

8. Immune-modulating effect

Methods of Ozone’s Therapeutic Effect 

Topical ozone therapy is the very fist method of applying ozone in the history of medical
practice. It should be noted that high concentrations are used for disinfection, while low
concentrations promote epithelialization and healing
External administration includes:

  • The use of ozonated antiseptic salines
  • Application of ozonated ointments and ozonated vegetable oil
  • Ozone aerated plastic bags under lowered pressure(Gas bags)
  • Balneotherapy

Parenteral methods include:

  • Major and Minor autohemotherapy with ozonized blood
  • Extracorporal plasma and lymph treatment
  • Subcutaneous ozone injections into biologically active points
  • Paravertebral intramuscular injections
  • Intravenous infusions of ozonated physiological solution

The method of intravenous infusions of ozonated physiological solution is the priority of the
Nizhny Novgorod School of Ozone therapy. It was developed and clinically tested in the
Central Research Laboratory of the Medical Academy of Nizhny Novgorod by Sergey
P.Peretyagin and Claudia N. Kontorschikova. The method was recognized as safe and easy to

In 1997, Professor Sergey D. Razumovsky, a well-known chemist, completed the investigation
of ozone effect on standard isotonic physiologic solution.

It was found that with the use of ozone neither chemical reactions take place in the solution,
nor new compounds (type of chlorine active derivatives) are accumulated.
Enteral method of ozone oxygen mixture is recommended in gastro-intestinal pathology. It

  • Intake of ozonated distillated water per os;
  • Intestinal irrigation with ozonated distilled water
  • Rectal insufflations with ozone oxygen mixtures


  • (1)– Oleg V. Maslennikov, Claudia N. Kontorshchikova, Irina A. Gribkova- Nizhny Novgorod, Russia, 2008
  • Ozone therapy in Practice. Health Manual. – Nizhny Novgorod
  • Rilling S.,Viebahn R. The Use of Ozone in Medicine. New Jork. Haug. 1987
  • Viebahn R. The use of ozone in medicine. 2nd ed.Heidelberg: Karl F. Haug

Discover the benefits of the ozone.